Diversification of inflammatory outcomes in human macrophages via alternate transcription in the...

...Toll-like Receptor signalling
Christine Wells
Afternoon Session, 3 September (11th MGED Meeting, 1-4 September, 2008)

Macrophages play a central role in pathogen detection and innate immune signaling. They have an impact on susceptibility to infection, tissue damage, wound healing, acute infection and chronic inflammatory disease. They look at subcellular localization, pathogen recognition/uptake, kinase activity, cytokine production, antigen destcruction and presentation. 256 variant transcripts were expressed from 70 genes in mouse bone-marrow-derived macrophages. These are predicted to generate decoy receptors, among other things. Use FIRMA analysis, which allows for variations in probe efficency, giving statistical analysis of gene-level and exon-level expression differences. Donor differences drive expression of Human TOLLIP isoforms: they are temporally regulated, but are variable between donors. Predictable expressin of some variants but stochastic expression of others may underlie phenotypic heterogeneity.

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